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Identification of Phytoconstituents of Tragia Involucrata leaf Extracts and Evaluate their Correlation with Anti-inflammatory & Antioxidant Properties

[ Vol. 20 , Issue. 3 ]

Author(s):

Vinodhini Velu, Swagata Banerjee, Vidya Radhakrishnan, Gaurav Gupta, Dinesh Kumar Chellappan, Neeraj Kumar Fuloria, Shivkanya Fuloria, Meenu Mehta, Kamal Dua* and Himaja Malipeddi*   Pages 308 - 315 ( 8 )

Abstract:


Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts.

Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using Soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be a major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract.

Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28-epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine- 1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87%) and H2O2 method (73.58%) at 200 μg mL-1. Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study.

Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent anti- inflammatory property.

Keywords:

Tragia involucrata, terpenoids, antioxidant, anti-inflammatory, GC-MS analysis, molecular docking.

Affiliation:

Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, VIT School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore- 632014, Tamil Nadu, School of Pharmacy, Suresh Gyan Vihar University, Jagatpura-302017, Mahal Road, Jaipur, Department of Life Sciences, School of Pharmacy, International Medical University, 57000 Bukit Jalil, Kuala Lumpur, Pharmaceutical Chemistry Unit, Faculty of Pharmacy, AIMST University, Bedong, Kedah, Darul, Aman, Pharmaceutical Chemistry Unit, Faculty of Pharmacy, AIMST University, Bedong, Kedah, Darul, Aman, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara- 144411, Punjab, Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney (UTS), Ultimo, NSW, 2007, Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore-632014, Tamil Nadu

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