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New Inhibition Detection Method to Evaluate the Human Salivary Alpha-amylase Activity of Some Drugs, Molecular Docking, and SAR Studies

Author(s):

Nia Samira*, Benarous Khedidja, Lakaas Manel, Sadeki Israa and Yousfi Mohamed   Pages 1 - 13 ( 13 )

Abstract:


Background: For the first time, the investigation of six anti-inflammatory drugs and six antihistaminic drugs for inhibitory activities against alpha-amylase has been evaluated using a new inhibition detection method in order to find new treatments for some diseases caused by α-amylase.

Objective: The first part of this work was devoted to the evaluation of the inhibition activity of these drugs on salivary α-amylase in vitro. Then to study the nature of interactions and structure-activity relationship, using Autodockvina program for molecular docking.

Method: The evaluation of the inhibitory activity of our drugs is achieved using a new method that has proved its sensitivity, quickness and effectiveness.

Results: The results of this study show that the betamethasone and loratadine are potent α-amylase inhibitors with IC50 values 0.7mg/ml and 1.03 mg/ml, respectively compared to acarbose with IC50=5.6 µg/ml.

Conclusion: The results showed that the loratadine and the betamethasone have a strong potential to inhibit the alpha amylase.

Keywords:

Inhibition activity, Human salivary α-amylase, Anti-inflammatory drugs, Antihistaminic drugs, Molecular docking

Affiliation:

Fondamontal science laboratory, Faculty of sciences, Amar Telidji University, Laghouat, Fondamontal science laboratory, Faculty of sciences, Amar Telidji University, Laghouat, Department of biology, Faculty of sciences, Amar Telidji university, Laghouat, Department of biology, Faculty of sciences, Amar Telidji university, Laghouat, Fondamontal science laboratory, Faculty of sciences, Amar Telidji University, Laghouat



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