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Inflammatory and Oxidative Stress Markers as Indicator of Atherogenesis in Rats: Antioxidants as Preventive Pharmacological Methods

[ Vol. 16 , Issue. 2 ]

Author(s):

Maria del Carmen Baez*, Mariana Taran, Maria de La Paz Scribano, Ariel Balceda, Carla Buonanotte, Sergio Blencio, Ismael Fonseca and Monica Moya   Pages 87 - 93 ( 7 )

Abstract:


Objective: The oxidative process in atherogenesis generated by proinflammatory induction and response to antioxidants vitamins in an experimental model were analyzed.

Methods: Male rats were used: (A)Control, (B)Control+vitamin E plus C, (C)Hyperfibrinogenemia and (D)Hyperfibrinogenemia+vitamins E plus C. Hyperfibrinogenemia induced by daily injection of adrenaline (0.1mg/day/rat) for 120 days. Treatment: 3.42 mg/kg of vitamin E plus 2.14 mg/kg of vitamin C, fifteen days after induction. Vascular histology analyzed by optical microscopy. Fibrinogen, nitrites and superoxide dismutase analyzed by spectrophotometry. Statistics: MANOVA, Hotelling test for post testing, significance level p<0.05.

Results: (C) group showed higher fibrinogen than (A) and (B)(p<0.001). Compared to (C) group, (D) showed a decrease of fibrinogen (p<0.001). A marked increase in nitrites was found in (C) versus (A), (B) and (D) groups (p<0.001). Superoxide dismutase activity increased in (C) group compared to groups (A) and (B) (p<0.001). In the group (D) an increase of the activity of this enzyme was observed in comparison to groups (C)(p<0.001), (A) and (B) (p<0.0001 in both). The (C) group shown endothelial denudation, thickening of the vascular intima and extracellular matrix enlargement with foam cells(p<0.001).

Conclusion: These results strongly suggest that vitamins E plus C produce regression of inflammatory and oxidative stress processes in this experimental model.

Keywords:

Antioxidants, atherogenesis, atherosclerosis, hyperfibrinogenemia, oxidative stress, vitamins E plus C.

Affiliation:

Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, II Catedra de Anatomia Patologica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba, Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba

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